Table 3.
Primers used for PCR detection of integrons and associated gene cassette regions.
| Integron | Primer | Sequence (5′–3′) | References |
|---|---|---|---|
| Class 1 | IntA | GTCAAGGTTCTGGACCAGTTGC | Rosser and Young, 1999 |
| IntB | ATCATCGTCGTAGAGACGTCGG | ||
| CASS1 | TGATCCGCATGCCCGTTCCATACAG | Rosser and Young, 1999 | |
| CASS2 | GGCAAGCTTAGTAAAGCCCTCGCTAG | ||
| 5′ CS | GGCATCCAAGCAGCAAG | Lévesque et al., 1995 | |
| 3′ CS | AAGCAGACTTGACCTGA | ||
| qacF | GGCTGGCTTTTTCTTGTTATC | Mazel et al., 2000 | |
| qacR | TGAGCCCCATACCTACAAAGC | ||
| Orf4 | CTAGCGAGGGCTTTACTAAGCTTGCC | Rosser and Young, 1999 | |
| Class 2 | Intl2F | GCAAATGAAGTGCAACGC | Mazel et al., 2000 |
| Intl2R | ACACGCTTGCTAACGATG |