Table 6.
Identification, integron structure, location of sul and dfr genes and plasmid content among sulfonamide- and/or trimethoprim-resistant strains.
| Strain | Farm | Source | Class of integron integrase | 3′-CS | Gene cassettes inside the Variable Zone (From 5′ to 3′) | sul and dfr genes outside the integron | Plasmid content | |
|---|---|---|---|---|---|---|---|---|
| Number | Size (kb) | |||||||
| Pseudomonas jessenii OT42 | F1 | Mucus | – | − | – | sul1 | 1 | 10 |
| Pseudomonas putida 0233 | F1 | Tank water | intI1 | + | aadA1 | – | 1 | 33 |
| Pseudomonas migulae Q11 | F2 | Tank water | intI1 | + | aadA1 | – | 0 | |
| Pseudomonas fluorescens Q20 | F2 | Fingerling | intI1 | + | – | – | 1 | 25 |
| Pseudomonas fluorescens Q23 | F2 | Fingerling | intI1 | + | aadA1 | – | 0 | |
| Pseudomonas syringae Q64 | F2 | Effluent | intI1 | + | – | – | 1 | 10 |
| Acinetobacer johnsonii Q67 | F2 | Pellet | intI1 | + | aadA1 | dfrA1, sul2 | 1 | 10 |
| Acinetobacer johnsonii Q75 | F2 | Pellet | intI1 | + | aadA1 | – | 1 | 30 |
| Pseudomonas fluorescens FP37 | C1 | Mucus | intI1 | + | aadA2, cmlA | dfrA12 | 1 | 10 |
| Pseudomonas fluorescens FP45 | C1 | Mucus | intI1 | + | aadA2, cmlA | dfrA12 | 1 | 10 |
| Pseudomonas fluorescens FP47 | C1 | Mucus | intI1 | + | aadA2, cmlA | dfrA12 | 1 | 30 |
| Citrobacter gillenii FP75 | C1 | Mucus | intI1 | + | dfrA12, orfF, aadA2a | dfrAl, sul2 | 1 | 10 |
| Kluyvera intermedia OP29 | C1 | Cage sediment | intI1 | + | dfrA12, orfF, aadA2 | sul2 | 1 | 10 |
| Pseudomonas baetica FE3 | C2 | Cage water | – | − | – | sul1 | 2 | 0.25/25 |
| Pseudomonas veronii FE4 | C2 | Cage water | – | − | – | sul1 | 0 | |
| Pseudomonas putida FF32 | C3 | Cage water | intI1 | − | – | – | 1 | 10 |
| Pseudomonas baetica SX52 | C3 | Mucus | intI1 | + | aadA9 | dfrA12 | 1 | 30 |
| Pseudomonas putida FB13 | C4 | Mucus | – | − | – | sul1 | 1 | 30 |
| Pseudomonas putida FB 15 | C4 | Mucus | intI1 | − | – | dfrA14, sul1 | 1 | 10 |
| Citrobacter freundii FB98 | C4 | Mucus | intI1 | + | dfrA12, orfF, aadA2 | dfrA14 | 1 | 3 |
| Pseudomonas fluorescens FR27 | C4 | Mucus | intI1 | + | aadA2, cmlA | dfrA12 | 2 | 10/40 |
| Pseudomonas baetica FR34 | C4 | Mucus | – | − | – | sul2 | 2 | 0.6/25 |
| Pseudomonas jessenii FR51 | C4 | Mucus | – | − | – | sul1 | 0 | |
| Pseudomonas arsenicoxydans SR72 | C4 | Intestinal content | – | − | – | sul1 | 1 | 25 |
| Sphingobacterium multivorum FM2 | C5 | Cage water | – | − | – | sul2 | 1 | 40 |
| Comamonas sp. FM3 | C5 | Cage water | intI1 | + | aadA2 | – | 2 | 1/10 |
| Pseudomonas syringae FM4 | C5 | Cage water | intI1 | + | – | – | 2 | 10/37 |
| Hafina sp. FM7 | C5 | Cage water | intI1 | − | – | – | 2 | 2.5/10 |
| Pseudomonas putida FM15 | C5 | Mucus | intI1 | − | – | sul2 | 1 | 30 |
| Pseudomonas putida FM22 | C5 | Mucus | – | − | – | sul1, dfrA14 | 1 | 10 |
3′ CS, 3′ conserved segment of class-1 integrons harboring the gene array qacEΔ1 + sul1.