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. 2019 Apr 12;10:465. doi: 10.3389/fpls.2019.00465

FIGURE 4.

FIGURE 4

Molecular identification of ZmDOF36-expressing transgenic maize plants. (A) Expression levels of ZmDOF36 in transgenic maize seedlings as determined by qRT-PCR. WT: wild-type (“ZZC01”). (B) Southern blot analysis of transgenic plants using the GUS gene as a probe. Genomic DNA was digested with EcoRI. M, λ/EcoRI DNA marker; +, plasmid DNA (pZZ00005-ZmDOF36) positive control. L3, L11, L16, and L17 are five independent transgenic lines that overexpress ZmDOF36.