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Analysis of the interaction between ZmDOF36 and the promoter regions of six starch synthesis genes in yeast one-hybrid assays. (A) Schematic diagrams of the bait and reporter constructs. (B) Screening on SD/-Leu medium containing 500 ng/mL Aureobasidin A (AbA); CK+, p53AbAi-p53 fragment+pGADT7-Rec (SmaI-linearized); CK-, p53AbAi+pGADT7-ZmDOF36; 1, pZmAGPS1a; 2, pZmAGPL1; 3, pZmGBSSI; 4, pZmSSIIa; 5, pZmISA1; 6. pZmISA3.
