FIGURE 5.

In vivo protein labeling analysis of plastid-encoded proteins. (A) Pulse labeling of chloroplast thylakoid proteins of WT and bfa2 mutants. Chloroplast thylakoid proteins were extracted from primary leaves of 12 days old seedlings after labeling with [³⁵S]-Met for 20 min, and separated by SDS-urea-PAGE. The labeled proteins were visualized using autoradiography. (B) Pulse-chase labeling of chloroplast stromal protein complexes of WT and bfa2-1 plants. After pulse labeling for 20 min (indicated as 0 min), the leaves were chased with cold Met for 15 and 30 min. Total soluble protein complexes extracted from the leaves were separated by CN-PAGE and the newly assembled complexes were detected by autoradiography.