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. 2019 Apr 12;10:751. doi: 10.3389/fmicb.2019.00751

Table 1.

Fusarium graminearum strains generated and used in this work.

Number Strain Resistance cassette Reference
1 WT 8/1 Miedaner et al., 2000
2 WT 8/1 GFP constitutive HygB Jansen et al., 2005
Single knock-out
3 ΔFghyd1 (FGSG_01763) HygB This study
4 ΔFghyd2 (FGSG_01764) NptII This study
5 ΔFghyd3 (FGSG_09066) Nat1 This study
6 ΔFghyd4 (FGSG_03960) HygB This study
7 ΔFghyd5 (FGSG_01831) HygB This study
Double knock-out
8 ΔFghyd23 Nat1/NptII This study
Triple knock-out
11 ΔFghyd123 Nat1/HygB/NptII This study
12 ΔFghyd234 Nat1/HygB/NptII This study
13 ΔFghyd235 Nat1/HygB/NptII This study
Single knock-out GFP constitutive
14 ΔFghyd1 GFP constitutive NptII/HygB This study
15 ΔFghyd2 GFP constitutive NptII/HygB This study
16 ΔFghyd3 GFP constitutive Nat1/HygB This study
17 ΔFghyd4 GFP constitutive HygB/NptII This study
18 ΔFghyd5 GFP constitutive HygB/NptII This study

HygB, Hygromycin; Nat1, Nourseothricin acetyltransferase; NptII, Neomycin phosphotransferase.