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. Author manuscript; available in PMC: 2019 Apr 19.
Published in final edited form as: Cell Rep. 2018 Jul 24;24(4):861–872.e6. doi: 10.1016/j.celrep.2018.06.045

Figure 4. batVP35 Does Not Inhibit PKR Phosphorylation or miRNA.

Figure 4

Silencing (A) Analysis of PKR phosphorylation in HEK293T cells in the presence of FLAG-tagged mVP35, eVP35, eVP35 R312A, and batVP35 (500 ng). Western blots were performed for total and phos- phorylated PKR. Three replicates were performed. E refers to empty vector control. (B) Analysis of miRNA silencing in HEK293T cells transfected with the indicated reporter, miRNA expression, and VP35 expression plasmids as indicated. Error bars represent the SEM for triplicate experiments. VP35 expression was assessed by western blot for the FLAG epitope tag. Statistical significance was assessed using a one-way ANOVA and Tukey’s test: ****p < 0.0001. The miR30-Luc luciferase reporter assay was repeated twice.