Table 3.
Results of SAXS analysis on the Ca2+-CaM•TR complex
| Concentration (mg/mL) | 1.9 | 6.4 |
|---|---|---|
| Guinier Analysis | ||
| Io/c | 1.34±0.01 | 1.26±0.00 |
| RG (Å) | 35.5±0.4 | 34.7±0.4 |
| qmin | 0.01234 | 0.01665 |
| qmaxRG | 1.25 | 1.27 |
| Molecular Mass Analysis (Da) | ||
| MW from QP (qmax) | 76955 (0.1971) | 74302 (0.2185) |
| MW from Vc (Vc; qmax) | 68580 (548; 0.3011) | 66871 (534;0.3011) |
| Bayesian credibility interval (MW) | 67900–75300 (72400) | 69650–75300 (72400) |
| P(r) Analysis | ||
| RG (Å) | 37.45±1.36 | 36.53±1.29 |
| Dmax (Å) | 130 | 128 |
| q range | 0.0195–0.2250 | 0.0238–0.2293 |
| χ2 | 0.8294 | 0.8360 |
| DAMMIF (20 runs, default parameters) | ||
| q range | 0.0195–0.2250 | 0.0238–0.2293 |
| Symmetry/anisometry | PI/unknown | PI/unknown |
| NSD | 1.384±0.183 | 1.166±0.113 |
| χ2 range | 0.765–0.767 | 0.971–0.977 |
| Resolution from SASRES (Å) | 42±3 | 37±3 |
| MM (0.5×Volume)(Da) | 69140±390 | 66870±315 |
| DAMMIN | ||
| q range | 0.0195–0.2250 | 0.0238–0.2293 |
| χ2 | 0.7928 | 0.9723 |