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. Author manuscript; available in PMC: 2019 Aug 14.
Published in final edited form as: Biochemistry. 2018 Jul 27;57(32):4788–4802. doi: 10.1021/acs.biochem.8b00542

Figure 4.

Figure 4.

UV-vis spectral monitoring of the reactions of ferric wt sw MbIII–H2O. (A) changes after addition of nitrite. (B) changes after addition of dithionite followed by the addition of nitrite as well as the plot of ΔAbs(421–457) against time. (C) UV-vis spectra of the three forms of wt sw Mb (ferric, deoxy ferrous, and NO) with the enlarged Q region. Conditions: 0.1 M phosphate buffer, pH 7.4, [protein] = 1.8–7.4 μM, [nitrite] in (A) = 40 mM, [dithionite] = 5 mM, [nitrite] in (B) and (C) = 4 mM.