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. 2019 Apr 12;10:447. doi: 10.3389/fpls.2019.00447

FIGURE 1.

FIGURE 1

Adhesion zones identified with fluorescent beads. (A) Chlorokybus (small arrow) adheres to glass or plastic substrates. Fluorescent beads label the zone adjacent to the cell attachment site (large arrow). Bar 5.2 μm. (B) Bead labeling of the cell wall surface of Penium (arrows) after EPS was washed from the cell surface. Bar 45.3 μm. (C) Bead labeling of the adhesive “footprint” (arrow) that remains on the substrate after Penium is washed from substrate surface. Bar 45.3 μm. (D) Rhizoids (arrows) emerge from live cells immediately adjacent to the wound zone in a wounded Spirogyra filament. Bar 21.1 μm. (E) Bead labeling of a sheath that is external to the cell wall of growing rhizoids (large arrow). Note that the surface of the rhizoid producing cell also labels (small arrows). (F,G) Labeled “clouds” of beads are found at the cross-wall areas of planktonic Zygnema filaments (arrows). (H,I) Bead labeling at the sheath surrounding the cell at a fragmentations site (arrow). (J) Bead labeling of the center thallus zones of Coleochaete (arrows). (K) The beads also label the thallus margins (K, arrow) and the hairs emerging from the dorsal side of the thallus (L, arrow). Bars (F–L) 5.2 μm.