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. 2019 Apr 12;10:447. doi: 10.3389/fpls.2019.00447

FIGURE 6.

FIGURE 6

Coleochaete: (A) Zoospores (arrows) that have adhered to the substrate. Bar 5 μm. (B) Vegetative cell (arrow) after zoospore settling and cell wall production. Bar 8 μm. (C) 96 h old vegetative multicellular thallus (arrow). Bar 12 μm. (D) Yariv reagent labeling of cell walls (arrows) at the periphery of the vegetative thallus. Bar 10 μm. (E) JIM13 labeling of zoospore cell walls (arrows). Bar 8 μm. (F) JIM13 labeling of the vegetative thallus (arrows). Bar 10 μm. (G) JIM13 labeling of the hairs (arrows) that emerge from the thallus. Bar 10 μm. (H) TEM view of the zoospore cell wall surface. Note the ordered packing of projections (arrows). Bar 300 nm. (I) JIM13 labeling of the zoospore cell wall. Labeling is found throughout the wall (arrows). Large arrows show the projection found in the outside of the cell wall of young Coleochaete. Small arrows represent JIM13. Bar 1 μm. (J) TEM image of an outer vegetative cell. Note the thick cell wall (arrow). Bar 2.25 μm. (K) TEM image of the vegetative cell wall. An inner wall layer of tightly packed fibers (large arrow) subtends a thin layer of fibrils (small arrow). Bar 450 nm. (L) JIM13 labeling of the cell wall of a vegetative cell. Note the labeling through the wall (arrows). Bar 500 nm. (M) JIM13 labeling of the hair cell wall. Bar 300 nm.