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. 2019 Apr 12;10:757. doi: 10.3389/fmicb.2019.00757

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Copyright © 2019 Haferkamp, Tjaden, Shen, Bräsen, Kouril and Siebers.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of PK and PEPS from Sul. solfataricus at 70°C. PK activity with PEP (A) and ADP (B) and PEPS activity with pyruvate (C) and ATP (D) was determined. Both enzymes follow classical Michaelis–Menten kinetics at 70°C. All measurements were performed in triplicate, the points in the figures are the mean values and the error bars indicate the standard deviation. Kinetic parameters are given in Tables 1, 2.