Table 1.

Kinetic parameters of the Sul. solfataricus PK for PEP and ADP determined at 50, 65, 70, and 80°C.

	Temp	Vmax	Kcat	Km	Kcat/Km
Substrate	[°C]	[U/mg]	[s-1]	[mM]	[mM-1s-1]
PEP	50	47.9	39.7	0.23	137.3
	65	60.4	50.1	0.12	435.8
	70	70.4	58.4	0.09	628.0
	80	88.7	73.0	0.26	281.0
ADP	50	48.3	40.1	0.16	244.3
	65	63.7	52.9	0.14	385.8
	70	70.8	58.8	0.07	851.9
	80	96.7	80.3	0.11	710.5

For the final enzyme characterization one enzyme preparation was used and the enzyme assays were performed in triplicates. The mean values and standard deviation is shown in Figure 2 and Supplementary Figures 1, 3.