Table 2.

Kinetic parameters of Sul. solfataricus PEPS for pyruvate and ATP determined at 65 and 70°C, respectively.

	Temp	Vmax	Kcat	Km	Kcat/Km
Substrate	[°C]	[U/mg]	[s-1]	[mM]	[mM-1s-1]
Pyruvate	65	0.27	0.41	0.54	0.75
	70	0.32	0.48	0.46	1.04
ATP	65	0.17	0.25	0.47	0.53
	70	0.22	0.33	0.61	0.53
PEP + AMP	70	0.03	0.05	ND	ND

For the final enzyme characterization one enzyme preparation was used and the enzyme assays were performed in triplicates. The mean values and standard deviation is shown in Figure 2 and Supplementary Figures 1, 3. ND, not determined.