Table 3.
Kinetic parameters of enzymes involved in PEP-pyruvate conversion in the archaeal species Tpt. tenax, Pyr. furiosus and Sul. solfataricus.
| Assay | |||||
|---|---|---|---|---|---|
| Organism | Enzyme | Temperature | Vmax | Km | Reference |
| Tpt. tenax | PEPS (anabolic) | 70°C | 0.45 | 0.4 (pyruvate) | Tjaden et al., 2006 |
| 1.0 (ATP) | |||||
| PEPS (catabolic) | 70°C | Not detectable | |||
| PPDK (anabolic) | 70°C | 1.1 | 0.8 (pyruvate) | ||
| 8.0 (ATP) | |||||
| PPDK (catabolic) | 55°C | 2.5 | 0.5 (PEP) | ||
| 0.02 (AMP) | |||||
| PK | 50°C | 46 | 0.7 (PEP) | Schramm et al., 2000 | |
| Pyr. furiosus | PEPS (anabolic) | 80°C | 14.9 | 0.11 (pyruvate) | Hutchins et al., 2001 |
| 0.39 (ATP) | |||||
| PEPS (catabolic) | 50°C | 2.5 | 0.4 (PEP) | ||
| 1.0 (AMP) | |||||
| Sul. solfataricus | PEPS (anabolic) | 70°C | 0.32 | 0.46 (pyruvate) | This study |
| 0.61 (ATP) | |||||
| PEPS (catabolic) | 70°C | 0.03 | ND | ||
| PK | 70°C | 70.4 | 0.09 | ||
| PPDK | 70°C | Not detectable | |||