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. Author manuscript; available in PMC: 2020 Feb 1.
Published in final edited form as: Biomarkers. 2018 Dec 12;24(1):76–90. doi: 10.1080/1354750X.2018.1508308

Figure 3. Verification of candidate circulating miRs by stem-loop qRT-PCR on MDI dermal exposed mice.

Figure 3.

Experimental timelines showing MDI exposed time points, sample collection and analysis in additional separate groups of mice (A). Serum total RNA were isolated by miRVana™ PARIS™ isolation kit, reverse transcribed, preamplified and subjected to TaqMan miR stem-loop qRT-PCR. Circulating miR expressions of candidate (B) mmu-miR-183-5p, (C) mmu-miR-206-3p, (D) mmu-miR-381-3p, (E) mmu-miR-127, (F) mmu-miR-192-3p, (G) mmu-miR-181a-1-3p, (H) mmu-miR-433-3p, (I) mmu-miR-744-3p, (J) mmu-miR-30d and (K) mmu-miR-153-3p changes were determined 4 days following 1% MDI/ACN exposure (N=3; bars, s.e.m). MDI: 4,4’-methylene diphenyl diisocyanate; Veh: Vehicle; ACN: acetone. (*P<0.05; **P<0.01).