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. 2019 Apr 19;14(4):e0215605. doi: 10.1371/journal.pone.0215605

Fig 1. Antibody responses to antigens in each steer.

Fig 1

Data trends were fit by Loess (degree = 2, smoothing = 0.3), and original data are shown as symbols. The solid gray lines indicate positive cut-off values as suggested by the kit manufacturer for each respective ELISA kit, and the dotted grey line indicates the positive cut-off value as calculated for the geographical region. Steers 1 (blue) and 2 (red) (top) were WT-BoHV-4- infected (wt +) and steers 3 (green) and 4 (brown) (bottom) were immunized only with BoHV-4-A-CMV-IgK-gE2gD-TM (wt -). WT-BoHV-4 infection is indicated in the first 24 weeks, immunization with BoHV-4-A-CMV-IgK-gE2gD-TM occurred at week 24, and dexamethasone-induced suppression took place during week 77. Panel A. BoHV-4 antibody percent positivity as determined by indirect ELISA. Panel B. BVDV antibody response as determined by VN. Panel C. BoHV-1 antibody response as determined by indirect ELISA. Panel D. BoHV-1 antibody response as determined by VN.