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. 2019 Apr 19;14(4):e0215387. doi: 10.1371/journal.pone.0215387

Fig 4. Epithelial differentiation is accelerated in fluid-supplemented mice.

Fig 4

A) Anpep mRNA expression levels (n = 4; *P<0.05 for fluid vs control on day 12, as determined by two-way ANOVA). B) Muc-2 mRNA expression levels (n = 4; **P<0.01 for fluid vs control on day 12, as determined by two-way ANOVA). C) ChgA mRNA expression levels (n = 4). D) Goblet cell count per crypt on day 12 (n = 9; **P<0.01 for fluid vs control, as determined by two-tailed t-test). E) Representative micrographs showing increased number and maturation of Goblet cells in the crypts of fluid-treated mice. Scale bars = 50 μm. F) In vivo permeability assay as determined by the presence of orally gavaged FD4 in plasma (n = 6–10). ANOVA detected a significant difference in “Time” factor (P<0.05).