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. Author manuscript; available in PMC: 2019 Jul 1.
Published in final edited form as: J Alzheimers Dis. 2019;67(3):1089–1106. doi: 10.3233/JAD-180923

Fig. 2.

Fig. 2.

Y10 enhances AβPP phosphorylation via cKit. A) IP-WB analysis of the effects of Y10 at 1 μM on AβPP phosphorylation of empty vector or cKit-transfected AβPP stable cells. B) Statistical analysis of (A). Results normalized to AβPP input. C) IP-WB analysis of the effects of Y10 on inhibition of cKit phosphorylation. D) Statistical analysis of (C). Results normalized to cKit input. E) In addition to Y10, a specific inhibitor of cKit, AB1010, also enhances AβPP phosphorylation on Tyr residue. F) Statistical analysis of (E). Results normalized to AβPP input. p-Y, phosphorylated Tyr. G, H) IP-WB analysis of the dose-dependent effects of Y10 and AB1010 on AβPP phosphorylation of cKit-transfected HEK cells stably expressing AβPP. I) Y10 and AB1010 were assayed at 1 μM for 18 h for their effects on Aβ40 level, as measured by ELISA in HEK293 cells stably overexpressing AβPP751. N=17 for Y10 and N=8 for AB1010. Standard error indicated. J) IP-WB analysis of the effects of Y10 at 1 μM on AβPP phosphorylation of control siRNA or cKit siRNA transfected HEK293 cells stably expressing AβPP. K) Statistical analysis of J for cKit intensity. L) Statistical analysis of G for AβPP phosphorylation. Results are mean ± standard deviation, n=3. Asterisks (*) indicate statistical significance of p<0.05 by t-test.