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. Author manuscript; available in PMC: 2019 Jul 1.
Published in final edited form as: J Alzheimers Dis. 2019;67(3):1089–1106. doi: 10.3233/JAD-180923

Fig. 4.

Fig. 4.

Y10 inhibits autophosphorylation of both cKit isoforms. A) cKit signaling assay in HEK293 cells. cKit GNNK+ and GNNK− isoforms were transfected into HEK293 cells and 48 h post-transfection cells were treated with SCF as indicated for 15 min, and cell lysates were collected for WB analysis with the antibodies indicated. B) IP-WB analysis of the effects of Y10 at 1 μM on cKit phosphorylation of GNNK+ or GNNK− isoforms-transfected HEK293 cells. Cells were treated with Y10 for 90 min and then 200 ng/mL of SCF for 15 min, and cell lysates were collected for IP-WB analysis. C) Statistical analysis of (B). Results normalized to cKit input. p-Y, phosphorylated Tyr. Results are mean ± standard deviation, n=3. Asterisks (*) indicate statistical significance of p<0.05 by t-test.