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. 2001 Nov 27;98(25):14714–14719. doi: 10.1073/pnas.251388798

Figure 6.

Figure 6

Pharmacological blockade of KCC3 activity by DIOA changes the phosphorylation of Rb and cdc2 kinase in KCC3-transfected cells. (A and C) Western blot analysis of phospho-Rb and phospho-cdc2 after 3-day incubation without or with 20 μM DIOA in KCC3 (A) or mock-transfected (C) cells. All Western blots first probed with phospho-Rb or phospho-cdc2 antibodies were stripped and reprobed with β-actin antibody as the internal standard. (B and D) Phosphorylated levels of Rb or cdc2 kinase were analyzed by scanning densitometry with β-actin as the internal standard. Results were expressed as arbitrary units. Each bar represents mean ± SEM (n = three independent experiments). *, P < 0.05, compared between groups in the absence or presence of DIOA.