Table 2.

Summary of binding affinities and kinetics of Affimer proteins F5 and G2 studied by SPR

	Fibrinogen			Fibrin
	KD ± SD (nM)	ka ± SD (M−1⋅s−1)	kd ± SD (s−1)	KD ± SD (nM)	ka ± SD (M−1⋅s−1)	kd ± SD (s−1)
F5	52 ± 1.3	3.6 ± 1.3 ×105	0.017 ± 0.002	58 ± 9.3	4.0 ± 0.6 ×105	0.023 ± 0.007
G2	38 ± 6.1	2.7 ± 0.5 ×105	0.010 ± 0.001	53 ± 8.2	2.6 ± 0.7 ×105	0.013 ± 0.003

Affinity and rate constants for association (k_a) and dissociation (k_d) were determined using 1:1 Langmuir binding model. Values are means ± SD of 3 independent experiments.