Skip to main content
. Author manuscript; available in PMC: 2019 Jun 19.
Published in final edited form as: Oncogene. 2018 Dec 19;38(16):2967–2983. doi: 10.1038/s41388-018-0608-2

Figure 5. Androgen receptor interrupts ARF/MET axis.

Figure 5.

(A) AR inversely correlates with MET in prostate cancer cell lines. ARF levels were used as reference control based on our previous report (20). (B) ARF, MET and its signaling proteins β-Catenin, SOX9 co-upregulation by androgen depletion in LAPC4 cells. LAPC4 cells were cultured in cFBS to deplete androgen for 6 days and regular FBS as control. (C) AR inversely correlates with MET in the prostate sphere. (D) ARF knockdown mediated MET downregulation is cytosolic MET-dependent. C, cytosolic lysates; N, nuclear lysates. (E) ARF knockdown mediated MET downregulation is androgen-dependent and is interrupted by AR activation. (F) AR overexpression and activation disrupts ARF and MET interactions. PC3 cells were transfected by AR and stimulated by DHT at 15nM for 24hrs. (G) ARF inducible expression in LNCaP cells increases androgen independent cell growth. GFP fusion ARF expression is detected by GFP expression by imaging (left panel) and cell growth were detected by crystal violet staining (right panel). (H) Nuclear MET stimulates LNCaP cells migration independent on androgen but mMET dependents on androgen. (I) AR correlates with DNA repair protein BRCA1 in nucleus. (J) ARF knockdown increases BRCA1 nuclear localization. (K) AR, nMET, and ARF regulate ATM and PARP but not caspase 3 investigated by western blot. Data are representative of averages+ SD (standard deviation).