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. 2019 Apr 18;26(4):584–592.e6. doi: 10.1016/j.chembiol.2019.01.003

Figure 1.

Figure 1

Comparison of Golgi Labeling with HaloTag and SNAP-tag Fusion Proteins of Sialyltransferase

(A) Top: Scheme of the labeling procedure. Bottom: Confocal images of live HeLa cells that have been treated as described in the scheme above. The white arrowheads indicate cells that express ManII-GFP and have been labeled with SiR-CA or SiR-BG. Scale bar: 20 μm.

(B) Quantification of cells expressing ManII-GFP that are positive for SiR from three independent experiments (ST-Halo, 740 cells in total; ST-SNAP, 837 cells in total).

(C) Fluorescence intensity distribution of HeLa cells that were incubated with SiR-CA or SiR-BG and that are transiently expressing ST-Halo-HA, ST-SNAP-HA, or no fusion protein. The number of cells (n) analyzed is indicated in the plot.