Figure 2.

Investigation of Various Factors that Could Cause a Difference in Labeling Using SNAP-tags or HaloTags

(A) Scheme of labeling procedures used in (B)–(D).

(B) Plot showing the percentage of cells expressing ManII-GFP that have also been immunolabeled with an antibody against the HA tag in three independent experiments (ST-Halo-HA, 463 cells in total; ST-SNAP-HA, 489 cells in total).

(C) Comparison of labeling efficiency of live and permeabilized cells using SNAP-tags and HaloTags from three independent experiments (ST-Halo-HA: live, 740 cells in total; fixed and permeabilized, 456 cells in total. ST-SNAP: live, 837 cells in total; fixed and permeabilized, 542 cells in total).

(D) Average fluorescence intensity of immunostained cells as described in (A).

(E) Intensity distribution in Drosophila egg chambers that are expressing Halo-SNAP-aPKC and have been labeled with SiR-CA or SiR-BG.