REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, Peptides, and Recombinant Proteins
SiR-HaloTag ligand (SiR-CA)	Promega	N/A (gift)
SiR-SNAP-tag ligand (SiR-BG)	Lukinavicius et al., 2013	N/A (gift)
TMR-HaloTag ligand (TMR-CA)	Promega	Cat# G8251
TMR-SNAP-tag ligand /SNAP-Cell® TMR-Star (TMR-CP)	NEB	Cat# S9105S
JF646-HaloTag ligand (JF646-CA)	Grimm et al., 2015	N/A
JF646-SNAP-tag ligand (JF646-BG)	Grimm et al., 2015	N/A
JF549-HaloTag ligand (JF549-CA)	Grimm et al., 2015	N/A
JF549-SNAP-tag ligand (JF549-BG)	Grimm et al., 2015	N/A
MeSiR-HaloTag ligand (MeSiR-CA)	Lukinavicius et al., 2013	N/A
MeSiR-SNAP-tag ligand (MeSiR-BG)	Lukinavicius et al., 2013	N/A
Experimental Models: Cell Lines
HeLa	ATCC	ATCC CCL-2
Experimental Models: Organisms/Strains
Cas9 expressing fly line CFD2	Port et al., 2015	N/A
Oligonucleotides
Primers and gBlock Used for Site-Directed Mutagenesis	This paper	N/A
Software and Algorithms
Prism Graphpad	Graphpad	Graphpad.com
Pymol	Schrödinger	https://www.schrodinger.com/pymol
UCSF Chimera	Pettersen et al., 2004	N/A
YASARA	Krieger and Vriend , 2015	N/A