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. Author manuscript; available in PMC: 2020 Apr 18.
Published in final edited form as: Cell Chem Biol. 2019 Feb 14;26(4):593–599.e4. doi: 10.1016/j.chembiol.2019.01.004

Figure 1.

Figure 1.

Most validated hits inhibit CYP51 or EBP and accumulate 8,9-unsaturated sterols in OPCs. A. Schematic representation of screening strategy. B. Dot-scattered plot of percentage of MBP+ oligodendrocytes generated after 72 h of treatment. Retested molecules are green. C. Heat-map representing percentage of MBP+ oligodendrocytes generated in two derivations of OPCs after treatment with top hits. D. Structure of CYP51 or EBP inhibitors identified. See Figure S1 for remaining hits. E, F GC-MS-based quantification of lanosterol (E) and zymostenol (F) after treatment with indicated molecules. In experiments C, E and F, n = 2 replicates per condition. All treatments 10 μM. See also Figure S1.