KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial Strains
Saccharomyces cerevisiae DBY745	Worland and Wang, 1989	N/A
Chemicals and Recombinant Proteins
mPEG-Succinimidyl Valerate, MW5000	Laysan Bio Inc.	mPEG-SVA-5000
Biotin-PEG-SVA, MW5000	Laysan Bio Inc.	Biotin-PEG-SVA-5000
Streptavidin	Thermo Fisher	S888
Cy3/Cy5 NHS Ester	GE Healthcare	PA13101 / PA15101
D-(+)-Glucose	Sigma	G7528-250G
Trolox	Sigma	238813-1G
Glucose oxidase	Sigma	G2133-50KU
Catalase	Sigma	C100-50MG
Oligonucleotides
Yclv1 for enzyme assay AGCGGTATCAGCAGGATGACGATGCGCGCATCGTCATCCTACAGAATCAGTTTTTTTTTTTTTTTT	Integrated DNA Technology (IDT)	N/A (custom)
Yclv2 for enzyme assay AGCGGTATCAGCAATCTAACAATGCGCGCATTGTTAGATTACAGAATCAGTTTTTTTTTTTTTTTT	Integrated DNA Technology (IDT)	N/A (custom)
Yclv3 for enzyme assay AGCGGTATCAGCAGGATGACGATGAGCTCATCGTCATCCTACAGAATCAGTTTTTTTTTTTTTTTT	Integrated DNA Technology (IDT)	N/A (custom)
Yclv4 for enzyme assay AGCGGTATCAGCAGGATAACGATGAGCTCATCGTTATCCTACAGAATCAGTTTTTTTTTTTTTTTT	Integrated DNA Technology (IDT)	N/A (custom)
Yclv5 for enzyme assay AGCGGTATCAGCAGGATAACAATGAGCTCATTGTTATCCTACAGAATCAGTTTTTTTTTTTTTTTT	Integrated DNA Technology (IDT)	N/A (custom)
Yclv6 for enzyme assay CCCTTTCCTTCATCGATAAGCTTTAATTAAAGCTTATCGATGAAAAGGAATTTTTTTTTTTTTTTT	Integrated DNA Technology (IDT)	N/A (custom)
Complementary oligonucleotides for sequences above, See Table S2	Integrated DNA Technology (IDT)	N/A (custom)
Oligonucleotides for cyclization assay, See Table S2	Integrated DNA Technology (IDT)	N/A (custom)
Software and Algorithms
IDL	Exelis Visual Information Solutions	https://www.harrisgeospatial.com/Software-Technology/IDL
Matlab R2017b	Mathworks	https://www.mathworks.com/products/matlab.html
OriginPro 9.1	OriginLabs	https://www.originlab.com/Origin
smFRET data acquisition program	Taekjip Ha (Johns Hopkins Univ.)	http://ha.med.jhmi.edu/resources
IDL scripts (custom)	This work	N/A
Matlab scripts (custom)	This work	N/A