| Sequence (5′−3′) | |
|---|---|
| T7 tagged forward primers for oligos 1–5 (PTBP1) | |
| PT_1 T7 F LY | TAATACGACTCACTATAGGGCGTATCTGCTTGCGTTGAC |
| PT_2 T7 F LY | TAATACGACTCACTATAGGCACCAGCAAGGAAAGCCTC |
| PT_3 T7 F LY | TAATACGACTCACTATAGCGATCTCAACTCACTGCAACCTC |
| PT_4 T7 F LY | TAATACGACTCACTATAGAGGCTGGTCTCGAACTCCTG |
| PT_5 T7 F LY | TAATACGACTCACTATAGGCAAGCGTCTCTTAGCAACAGG |
| Reverse primers for oligo 1–5 (PTBP1) | |
| PT_1 R LY | GCTTTCCTTGCTGGTGCAGA |
| PT_2 R LY | CGGAGGTTGCAGTGAGTTGAG |
| PT_3 R LY | TGGGTGGATCACCTGAGGTC |
| PT_4 R LY | AAGAGACGCTTGCAGCCTAC |
| PT_5 R LY | GACAAACAGTGAGAGCAGAATAGC |
| Primers used for mutant construct (PTBP1) | |
| Forward primers | |
| T7 Mut PTBP1 Oligo 3 F | TAATACGACTCACTATAGCGATCTCAACTCACTGCAACCTC |
| T7 Mut PTBP1 Oligo 4 F | TAATACGACTCACTATAGAGGCTGGTCTCGAACTCCT |
| T7 Mut NOVA1 Oligo 3 F | TAATACGACTCACTATAGCGATCTCAACTaACTGCAACCTC |
| T7 Mut NOVA1 Oligo 4 F | TAATACGACTCACTATAGAGGCTGGTCTCGAACTCCT |
| Reverse primers | |
| Mut PTBP1 Oligo 3 R | TGGGTGGATCACCTGAGGTC |
| Mut PTBP1 Oligo 4 R | TTGTGTCGCTTGCAGCC |
| Mut NOVA1 Oligo 3 R | TtGGTtGATCACCTGAGGTC |
| Mut NOVA1 Oligo 4 R | AAGAGACGCTTGCAGCC |
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