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. Author manuscript; available in PMC: 2019 Dec 26.
Published in final edited form as: Biochemistry. 2018 Dec 12;57(51):7011–7020. doi: 10.1021/acs.biochem.8b00946

Figure 6.

Figure 6.

PA3944 activity with respect to polymyxin B in different buffers and at different pHs. (A) Comparison of polymyxin B substrate saturation curves at a constant concentration of AcCoA (0.5 mM) in Tris-HCl and Bis-Tris propane buffers at pH 8.0. Data were fit using nonlinear regression as described in Methods. (B) Polymyxin B substrate saturation curves as a function of pH in Bis-Tris propane buffers from pH 6.5 to 9.5. Data were fit using nonlinear regression as described in Methods. (C) PA3944 activity with respect to 2 mM polymyxin B and 0.5 mM AcCoA in Bis-Tris propane buffers at pH 6.5, 7.0, 8.0, 9.0, and 9.5. Data were fit to a Gaussian curve. See Methods for further details.