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. Author manuscript; available in PMC: 2019 Dec 26.
Published in final edited form as: Biochemistry. 2018 Dec 13;57(51):6964–6972. doi: 10.1021/acs.biochem.8b01092

Figure 2.

Figure 2.

(A) Region of the 1H-15N HSQC spectra of non-phosphorylated V118D KID (black) and CKII-phosphorylated V118D KID (pCKKID, red). The two cross peaks observed for pS117 arise from incomplete phosphorylation of T119 (pS117a - T119 not phosphorylated labeled in purple; pS117b - T119 phosphorylated, labeled in red). Assignments of non-phosphorylated and fully-phosphorylated KID are shown with black labels, assignments for partially phosphorylated species are indicated using the color code of Fig. S1B. (B) Region of the 1H-15N HSQC spectrum of CKII-phosphorylated full-length CREBD,4 in complex with unlabeled CRE DNA. T119 was fully phosphorylated in the full-length CREB sample.