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. Author manuscript; available in PMC: 2019 Dec 26.
Published in final edited form as: Biochemistry. 2018 Dec 13;57(51):6964–6972. doi: 10.1021/acs.biochem.8b01092

Figure 3.

Figure 3.

(A) Region of the 1H-15N HSQC spectrum of 15N-labeled pCKKID before (black) and after addition of unlabeled bZip (red) to a mole ratio of 1:1.125 pCKKID:bZip dimer. (B) Region of the 1H-15N HSQC spectrum of non-phosphorylated 15N-labeled KID before (black) and after addition of unlabeled bZip (red). The mole ratio is the same as in panel A. (C) Weighted average chemical shift changes for pCKKID residues resulting from addition of bZip dimer at 1:1.125 mole ratio. Red bar indicates the CK cassette. (D) Weighted average chemical shift changes for non-phosphorylated KID resulting from addition of bZip dimer at 1:1.125 mole ratio. Proline residues are indicated by a P below the x-axis. Black circles at the x-axis indicate residues that could not be assigned.