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. Author manuscript; available in PMC: 2019 Apr 20.
Published in final edited form as: Cell Rep. 2019 Feb 12;26(7):1774–1786.e4. doi: 10.1016/j.celrep.2019.01.077

Figure 2. Fur1 Heterozygosity Is Protective in LRRK2IT-Overexpressing Neurons.

Figure 2.

(A–D) Representative images of DA neurons stained with anti-TH antibody (red) from PPL1 clusters in 60 day-old female flies of the following genotypes: (A) UAS-LacZ (Ddc-Gal4/UAS-LacZ), (B) fur1+/− (Ddc-Gal4/+; fur1rl205/+), (C) UAS-LRRK2IT (Ddc-Gal4/+; UAS-LRRK2IT/+), and (D) UAS-LRRK2IT, fur1+/− (Ddc-Gal4/+; UAS- LRRK2IT/fur1rl205).

(E) Quantification of the number of TH-positive DA neurons in PPL1, PPM1-2, and PPM3 clusters for genotypes in (A)–(D). n = 22 hemispheres for each genotype. Data are represented as mean ± SEM. One-way ANOVA with Bonferroni post-test.

(F) Representative lifespan curves for genotypes in (A)–(D). n = 100 females for each genotype (see also Table S1). Log-rank and Wilcoxon tests.

(G) Climbing activity for genotypes in (A)–(D). n = 60 flies for each genotype. Data are represented as mean ± SEM. Two-way ANOVA with Dunnett post-test.

(H–K) Representative images of DA neurons stained with anti-TH antibody (red) from PPL1 clusters in 60 day-old female flies of the following genotypes: (H) UAS-LacZ (Ddc-Gal4/UAS-LacZ), (I) fur1+/− (Ddc-Gal4/+; fur1rl205/+), (J) UAS-S6KSTDE (Ddc-Gal4/+; UAS-S6KSTDE/+), and (K) UAS- S6KSTDE, fur1+/− (Ddc-Gal4/+; UAS- S6KSTDE/fur1rl205).

(L) Quantification of the number of TH-positive DA neurons in the PPL1, PPM1-2, and PPM3 clusters for genotypes in (H)–(K). n = 22 hemispheres for each genotype. Data are represented as mean ± SEM. One-way ANOVA with Bonferroni post-test.

(M–P) Representative images of DA neurons stained with anti-TH antibody (red) from PPL1 clusters in 60 day-old female flies for the following genotypes: (M) UAS-LacZ (Ddc-Gal4/UAS-LacZ), (N) UAS-LRRK2IT (Ddc-Gal4/+; UAS-LRRK2IT/+), (O) UAS-LRRK2IT, Fur1RNAi (Ddc-Gal4/+; UAS- LRRK2IT/UAS-Fur1-RNAi), and (P) UAS-LRRK2IT, mCherryRNAi (Ddc-Gal4/+; UAS- LRRK2IT/UAS-mCherry-RNAi).

(Q) Quantification of the number of TH-positive DA neurons in the PPL1, PPM1-2, and PPM3 clusters for genotypes in (M)–(P). n = 22 hemispheres for each genotype. Data are represented as mean ± SEM. One-way ANOVA with Bonferroni post-test.

*p < 0.05, **p < 0.01 ***p < 0.001.