(A–D) Representative images of DA neurons stained with anti-TH
antibody (red) from PPL1 clusters in 60 day-old female flies of the following
genotypes: (A) UAS-LacZ (Ddc-Gal4/UAS-LacZ), (B)
fur1+/− (Ddc-Gal4/+;
fur1rl205/+), (C) UAS-LRRK2IT
(Ddc-Gal4/+; UAS-LRRK2IT/+), and (D) UAS-LRRK2IT,
fur1+/− (Ddc-Gal4/+; UAS-
LRRK2IT/fur1rl205).
(E) Quantification of the number of TH-positive DA neurons in PPL1,
PPM1-2, and PPM3 clusters for genotypes in (A)–(D). n = 22 hemispheres
for each genotype. Data are represented as mean ± SEM. One-way ANOVA with
Bonferroni post-test.
(F) Representative lifespan curves for genotypes in (A)–(D). n =
100 females for each genotype (see also Table S1). Log-rank and Wilcoxon
tests.
(G) Climbing activity for genotypes in (A)–(D). n = 60 flies for
each genotype. Data are represented as mean ± SEM. Two-way ANOVA with
Dunnett post-test.
(H–K) Representative images of DA neurons stained with anti-TH
antibody (red) from PPL1 clusters in 60 day-old female flies of the following
genotypes: (H) UAS-LacZ (Ddc-Gal4/UAS-LacZ), (I) fur1+/−
(Ddc-Gal4/+; fur1rl205/+), (J)
UAS-S6KSTDE (Ddc-Gal4/+; UAS-S6KSTDE/+), and (K) UAS-
S6KSTDE, fur1+/− (Ddc-Gal4/+; UAS-
S6KSTDE/fur1rl205).
(L) Quantification of the number of TH-positive DA neurons in the PPL1,
PPM1-2, and PPM3 clusters for genotypes in (H)–(K). n = 22 hemispheres
for each genotype. Data are represented as mean ± SEM. One-way ANOVA with
Bonferroni post-test.
(M–P) Representative images of DA neurons stained with anti-TH
antibody (red) from PPL1 clusters in 60 day-old female flies for the following
genotypes: (M) UAS-LacZ (Ddc-Gal4/UAS-LacZ), (N) UAS-LRRK2IT
(Ddc-Gal4/+; UAS-LRRK2IT/+), (O) UAS-LRRK2IT,
Fur1RNAi (Ddc-Gal4/+; UAS- LRRK2IT/UAS-Fur1-RNAi), and
(P) UAS-LRRK2IT, mCherryRNAi (Ddc-Gal4/+; UAS-
LRRK2IT/UAS-mCherry-RNAi).
(Q) Quantification of the number of TH-positive DA neurons in the PPL1,
PPM1-2, and PPM3 clusters for genotypes in (M)–(P). n = 22 hemispheres
for each genotype. Data are represented as mean ± SEM. One-way ANOVA with
Bonferroni post-test.
*p < 0.05, **p < 0.01 ***p < 0.001.