KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| TH (1/250) | Novus biologicals | Cat# NB300-109; RRID:AB_10077691 Lot ajo125 g |
| TH (1/250) | Immunostar | Cat# 22941; RRID:AB_572268 Lot 1602001 |
| Furin1 (1/100) | Roebroek et al., 1993 | N/A |
| HA (1/100) | BioLegend | Cat# 901503; RRID:AB_2565005 Lot D14CF00556 |
| repo (1/250) | DSHB | Cat# 8D12 anti-Repo; RRID:AB_528448 |
| elav (1/250) | DSHB | Cat# ELAV 9F8A9; RRID:AB_231436 |
| elav (1/250) | DSHB | Cat# ELAV 7E8A10; RRID:AB_528218 |
| Smad3 (1/250) | Abcam | Cat# ab52903; RRID:AB_882596 Lot GR128879-73 |
| DAPI (1/1000) | Thermo Fisher Scientific | Cat# D1306 |
| Bacterial and Virus Strains | ||
| N/A | N/A | N/A |
| Biological Samples | ||
| N/A | N/A | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Methyl viologen dichloride hydrate | Sigma Aldrich | Cat#856177-1G |
| Mifepristone | Cayman chemical company | Cat#10006317 |
| Critical Commercial Assays | ||
| TUNEL assay (FragEL™ DNA fragmentation detection kit, fluorescent – TdT Enzyme) | Millipore Sigma | Cat#QIA39-1EA |
| iTaqTM univesral sybrR green supermix | Bio-Rad | Cat#172-5121 |
| iScript cDNA synthesis kit | Bio-Rad | Cat#170-8891 |
| Deposited Data | ||
| N/A | N/A | N/A |
| Experimental Models: Cell Lines | ||
| N/A | N/A | N/A |
| Experimental Models: Organisms/Strains | ||
| UAS-hLRRK2I2020T | Venderova et al., 2009 | FBal0244982 |
| UAS-hLRRK2G2019S | Gehrke et al., 2010 | FBal0249350 |
| UAS-dLRRKR1441G | D. Park | FBal0322854 |
| UAS-Gbb.GFP | R. Ray | BDSC_63058 |
| UAS-eGFP::Fur1 | This paper | N/A |
| UAS-TkvACT (UAS-tkvQ199D) | Haerry et al., 1998 | FBal0051065 |
| UAS-eGFP | This paper | N/A |
| UAS-Mad | McCabe et al., 2004 | N/A |
| UAS-S6KSTDE | Barcelo and Stewart, 2002 | BDSC_6913 |
| UAS-LacZ | Exelixis, Inc. | BDSC_8529 |
| UAS- 4E-BPAA | Teleman et al., 2005 | N/A |
| UAS-mCherryRNAi | Perkins et al., 2015 | BDSC_35787 |
| UAS-Fur1RNAi | Dietzl et al., 2007 | VDRC 22853 |
| UAS-MadRNAi | Perkins et al., 2015 | BDSC_31316 |
| UAS-tkvRNAi | Perkins et al., 2015 | BDSC_35653 |
| UAS-DppRNAi | Perkins et al., 2015 | BDSC_25782 |
| UAS-GbbRNAi | Perkins et al., 2015 | BDSC_34898 |
| fur1rl205 | Penney et al., 2016 | BDSC_10341 |
| MadK00237 | Li et al., 2005 | BDSC_10474 |
| MedeaC246 | Aberle et al., 2002 | FBal0156263 |
| tkv7 | Nellen et al., 1994 | BDSC_3242 |
| gbb1 | M.B. O’Connor | FBal0092973 |
| dLRRKe03680 | Parks et al., 2004 | FBal0185431 |
| gbb.HA | M.B. O’Connor | N/A |
| Ddc-Gal4 | Li et al., 2000 | BDSC_7010 |
| Ddc-Gal4 | Li et al., 2000 | BDSC_7009 |
| repo-Gal4 | Sepp et al., 2001 | BDSC_7415 |
| elav-GS | Osterwalder et al., 2001 | N/A |
| y1 w67c23 | B. Gelbart | BDSC_6599 |
| Canton S | Kyoto stock center | BDSC_64349 |
| y1; ry506 | G. Karpen | BDSC_4405 |
| w1118 | R. Levis | BDSC_3605 |
| UAS-Fur1-sensor | Penney et al., 2012 | N/A |
| Oligonucleotides | ||
| qPCR and sequencing primers, Refer to Table S2 | Integrated DNA technologies | N/A |
| Recombinant DNA | ||
| cDNA clone: LD33976 | DGRC | Stock#5309 |
| Plasmid: pTGW | DGRC | Stock#1075 |
| Plasmid: pUASt | Brand and Perrimon, 1993 | N/A |
| Software and Algorithms | ||
| Illustrator (CS6) | Adobe | RRID:SCR_014198; https://www.adobe.com |
| Photoshop (CS6) | Adobe | RRID:SCR_014199; https://www.adobe.com/ |
| Zen | Zeiss | RRID:SCR_01367; https://www.zeiss.com/microscopy/int/products/microscope-software/zen.html |
| FIJI is just ImageJ (v. 2.0.0) | NIH | RRID: SCR_003070; https://fiji.sc |
| Prism (v. 7) | GraphPad Software | RRID: SCR_002798; https://www.graphpad.com |
| Imaris (v. 7.5.2) | Bitplane | RRID:SCR_007370; http://www.bitplane.com |
| Other | ||
| N/A | N/A | N/A |