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. 2019 Apr 19;9:6316. doi: 10.1038/s41598-019-42838-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Hyperpolarized mitochondria in fenofibrate-treated MS1 VEGF angiosarcoma cells. Data were generated by flow cytometry in MS1 VEGF angiosarcoma cells using the mitochondrial membrane potential indicator dye JC-1. (a–d) Example experiment showing the effect of a 48-hour treatment with DMSO (Ctrl) or 50 μM fenofibrate on JC-1 red fluorescence (a,b). The mitochondrial depolarizing agent CCCP was used as a positive control (c). (d) Mean data for experiments exemplified in a-c (n = 4). ****P < 0.0001 (one-way ANOVA followed by Tukey’s multiple comparisons test).