Table 2.
Byssal proteins identified by LC-MS/MS analysis using the foot transcriptome library.
| Protein name | Protein Sequence (# of amino acids)a,b Protein variants molecular weight |
MW, pIa | Scorec (−10LogP) |
Spectral Matches (#) | |||
|---|---|---|---|---|---|---|---|
| TP | 6 kDa | 7 kDa | 14 kDa | ||||
| Dbfp1-f1 | WNDKYPGDGKDKYPGDGDDKYLGGGNDKYLGGVFDKYFGGGN (42) | 4.6 kDa pI 4.4 | 75.07 | 3 | 1 | 1 | 1 |
| Dbfp1-f2 | KYPGDGNPKYPGGGNPKYPGGGNPKYPGGGNPKYPGGGNAKYLGGGNDKYP (51) | 5.1 kDa pI 10 | 27.12 | 1 | 0 | 0 | 0 |
| Dbfp2 | MLSSVTLLFVACCGMALGQGNSWDSYRPYPVYTPKPSYPDYPEKPYPPKQTYPTYPEKKYPTYPEKKYPTYPEKKYPTYPEKKYPTYPEKKYPTYPEKKYPTYPEPTYPTYTEKKYPAYTPKTYPTYTEKKYPDYPEKKYPDYPEKKYPDYPEKKYPSYPEKKYPAYPPKNSYPGRYPWRR (164) | 20.2 kDa pI 9.7 | 90.13 | 16 | 4 | 6 | 4 |
| Dbfp4 | MFGLVAVSVFLFCHSSAFSNTWQNRIKQRPTPVVPFKLEWYLGKWFTQSRQEPCSWKGSADFENMELNFVLDPKKNILYDHSIWKKNNRCVFVTFDIIPSPKTPGTFLIQDPLGDIQSGEYVILAIDPCKFVVEWGCTKPSPIGQRCDDPWVSVHTRERPSPKVLAEVDLALMRTVGVRLAELPRLSHANTPCCLGEGKLIQHDFL (190) | 21.8 kDa pI 8.1 | 149.48 | 20 | 0 | 0 | 0 |
| Dbfb5 | MFSAVTLVLLVSCCGTALSQRNSYGNYRPVKPPGQPINQYNQYSNPYRPQYNQNWNPYRPEQAPRYPQQSYPAYPPKQPYPAYPTKQPYPTDPPKQPYPANPSKPSYPANPPYDPCDEVYCRPIYCPNGQYKPTGECCPQCQPGTYLPKPWSWRGQGNVVGEQEKFVGEGNVVGDQTYDVGGQGNVVGGQRNVVDGKGNVVGEQRNNVGG (190) | 21.3 kDa pI 8.7 | 85.71 | 3 | 1 | 0 | 0 |
| Dbfp5β variant MW:15.6 kDa, pI: 5.7 | |||||||
| Dbfp6 | MFSAASFLLLVMFCGTVTSQFYWGYLPQRLYPRDPCDDVDCRTPHCPNGGYIPIGQCCPKCKPAASWALEVTLHVFSGRPDPEYVIPRDTSAYDAILKAIGDTSTPLGERLGYNGFTVIQTHGDSEVSHWTVGWCTRPKVELRLLAAVSAMTPIGDQHPLQKEVIDTVKQSIMLCKV (154) | 17.6 kDa pI 6.6 | 110.57 | 9 | 1 | 1 | 1 |
| Dbfb7α | MFFAVTLVLLVSCSGTPLGKWDPYGSSYGNSYGRPYGKAFNPYNQYGNSYPQNNQKWNSYWPNYKQPWNSYGPQQYPSYPQSGSYYPGSWGWPGNNVGSQGNAVDGLWNVVGWQGNDVDGLGNNVGKQWNDVDGVGNYVGKQWNNVD (132) | 13.2 kDa pI 4.7 | 147.16 | 16 | 10 | 10 | 9 |
| Dbfb7α–Dbfb7ζ variants MW/pI: 14.8/6.6, 14.2/4.3, 13.2/4.7, 12.3/4.7, 10.3/8.7, 9.7/9.1 | |||||||
| Dbfp8β | MKLALLAVIAFVAPSCYEATYPVPNQGRCLKDGQYFASGHFVDPTNRCTSCECFPGGNYQCRRDACPALSCPVNQRFYPHDACCQRCHGVIHSPGSASSVSSSDHDTSGTSRHTSKSSKSSRGTSKNSKSSKKSSKSKSSRKGSKGSRKGSRKGSRKGYKKYGKKGSGSSS (152) | 16.3 kDa pI 10.6 |
68.3 | 3 | 0 | 0 | 0 |
| Dbfp8α variant MW,pI: 16.7 kDa, pI 10.7 | |||||||
| Dbfp9β | MNTKQLMCLLVAAALLLASAPAANARFVYGDYDDDYGYGGKYGYPGNYGYPGNSGYPGNYGGYGNYGDNDY*GGWLGGLLGGGGRGNKWGGNYGNYGYGK (75) | 7.9 kDa pI 4.6 | 106.28 | 9 | 9 | 7 | 1 |
| Dbfb9α variant MW,pI: 9.0 kDa, pI 4.3 | |||||||
| Dbfp10α | MQSAVTLLLLVSCCGMALGQWDDYDDWDWPTGYPSYPPKQSYPPYPPYDPCKNVNCIQVVCPYGEYTPPGKCCPVCIDWGWPWGPYGSSGSDDYDDDDDYWPYNWGK (88) | 10.3 kDa pI 3.5 | 101.96 | 4 | 0 | 0 | 0 |
| Dbfb10β and Dbfp10γ variants MW/pI (kDa/pH): 7.9/4.7, 6.6/8.6 | |||||||
| Dbfp11γ | MCSATPFLLLVTFCGAVSSLWYPDRPCYGKVCPAIYCLYGQVTPPGKCCPQCKPDPGSNVHVPCKKDKDCAYVVCENPGEKVECHDAPSSYPPRRECHCHIPEECEKDFDCVDECGPGATCDDGACHGNDCDHT (115) | 12.7 kDa pI 4.4 | 137.49 | 0 | 0 | 0 | 0 |
| Dbfb11α–Dbfp11ε variants MW/pI (kDa/pH): 13.1/5.5, 12.7/4.4, 12.5/4.9, 12.4/4.9 | |||||||
| Dbfp12δ | MALSTWSLFLIVAATMYTGSCQECPVGSILKGCEFIQGHEGPWCPVGYYCRDLMMNNLGICCRNVCWDGPPITDNYGRAIDCSRGRTGLCPGATECVRYGRYGARSFCCNIRVTIG (96) | 10.5 kDa pI 7.8 | 119 | 3 | 0 | 0 | 3 |
| Dbfp12α–Dbfp12δ variants MW/pI (kDa/pH): 10.9/8.3, 10.7/8.1, 10.7/8.1 | |||||||
| Dbfp13α | MKGVFLLLAIVCIMVEAGGRRNQRPMYRRRLPPPTTKKPPPRPTPAPTQAPRGGPYEHEQLTQNIEKQLKEMNTTLDAIYTLTNEMFTIRNKCLDAYRPSG (82) | 9.6 kDa pI 10.8 | 52.4 | 2 | 0 | 0 | 0 |
| Dbfp13α–Dbfp13ε variants MW/pI (kDa/pI): 9.6/10.8, 9.2/10.6, 8.3/10.2, 7.1/9.94, 7.1/10.0 | |||||||
| Dbfp14γ | MGPNKLFVTVLILCLMGAMGGSDDPPAAPGRCYCRRVCRPNECYVGRCPRRRSWSWCCHRSHPDCEGK (49) | 5.6 kDa pI 8.6 | 89.84 | 6 | 6 | 14 | 7 |
| Dbfp14α-Dbfp14δ variants MW/pI (kDa/pH): 6.6/8.2, 6.5/8.6, 5.6/8.6, 5.4/8.2 | |||||||
| Dbfp15α | MCLAAAVLLAIAPIANAKYGSSSDSSDSDGYNGKRGGYGRRGGLPWPRYGRGKGYGGWGDNYGAVPTYGK (52) | 5.5 kDa pI 9.9 | 69.24 | 2 | 0 | 2 | 1 |
| Dbfp15β variant MW/pI (kDa/pH): 5.0/9.0 | |||||||
| Dbfp16 | MFSAVVTVLIICLMGVMGGGDREPAGPDWWCDWRWQCSRNECVVFEDFGGKFCCLWTSPLCWSKDR (44) | 5.3 kDa pI 4.4 | 68.66 | 3 | 1 | 1 | 0 |
| Dbfp17 | MTSVRILVVLMVVCILAGSVVQQAEAQCHMTLKGCANNECFTGTVGKRKKCCPKKNNACPQGLPSV (44) | 4.3 kDa pI 9.2 | 47.92 | 2 | 0 | 0 | 0 |
Novel proteins have been named Dbfp4 – Dbfp17. Signal peptides are underlined and matching peptide spectra are indicated in bold. For proteins with isoforms, the transcript with highest number of observed spectra or the largest molecular weight is shown as a representative sequence. Proteins variants are listed beneath the representative sequence.
aSequence properties were calculated after removing the predicted signal peptide sequence.
bN and Q signify asparagine deamidation, and Y signifies tyrosine hydroxylation, respectively.
cPEAKS scoring method: a −10LogP score cut-off of 20 is equivalent to P-value of 0.01.