Table 1.
Allocation of the Animals in the Groups
| Methods | Groups | N | Comments | Figures |
|---|---|---|---|---|
| PET | Sham | 8 | Each rat was injected with 13N‐NH3 3 times and scanned 3 times. | 1B through 1D |
| 5 min | ||||
| 4 h | ||||
| Lectin‐FITC perfusion and H&E stain (LAD) | Sham | 6 | One rat developed vessel injury and bleeding following passage of the needle through the LAD; lectin‐labeled sections can be used for H&E staining without affecting H&E staining. | 2A through 2D; 5C through 5E |
| 20 sec | 6 | |||
| 5 min | 9 | |||
| 30 min | 6 | |||
| 1 h | 6 | |||
| 2 h | 6 | |||
| 4 h | 9 (−1) | |||
| 6 h | 6 | |||
| Lectin‐FITC perfusion and H&E stain (RCA) | Sham | 6 (−1) | One rat died of cardiac arrest. | 3A through 3D; 6A through 6E |
| 20 sec | 6 | |||
| 5 min | 6 | |||
| 30 min | 6 | |||
| 1 h | 6 | |||
| 2 h | 6 | |||
| 4 h | 6 | |||
| 6 h | 6 | |||
| Lanthanum nitrate perfusion | Sham | 5 | Samples are not stained. | 4A |
| 5 min | 5 | |||
| Immunofluorescence (collagen IV/ZO‐1/VE‐cadherin) | Sham | Immunofluorescence was performed on sections labeled by lectin‐FITC. | 4B through 4E | |
| 5 min | ||||
| TEM | Sham | 9 (−1) | A rat died of anesthesia. | 5F and 5G |
| 30 min | 9 | |||
| Enoxaparin treatment | Sham | 6 | One rat died of ventricular fibrillation. | 7A |
| 5 min | 6 (−1) | |||
| 4 h | 7 | |||
| Aspirin treatment | Sham | 6 | One rat died of ventricular fibrillation. | 7B |
| 5 min | 6 (−1) | |||
| 4 h | 7 | |||
| Cardiac function | Sham | 5 | 7C and 7D | |
| 5 min | 5 | |||
| 4 h | 5 | |||
| Blood gas | Sham | 5 | 7E through 7G | |
| 5 min | 5 | |||
| 4 h | 5 |
H&E indicates hematoxylin and eosin; LAD, left anterior descending coronary artery; PET, positron emission tomography; RCA, right coronary artery; TEM, transmission electron microscopy; VE‐cadherin, vascular endothelial cadherin; ZO‐1, zonula occludens‐1.