Figure 1.

HIF1α signaling in male and female hPASMCs. Representative western blot of HIF1α protein expression in male and female hPASMCs (A) and densitometric analysis (B). Representative western blots of PHD2 and FIH protein expression in male and female PASMCs (C) with densitometric analysis (D). Data are shown as mean±SEM. n=3 to 6; *P<0.05 as determined by a Student's unpaired t‐test. Membranes were cut at an appropriate point and probed for separate antibodies. Membranes were then stripped and re‐probed with β‐actin. FIH indicates factor‐inhibiting hypoxia‐inducible factor; hPASMC, human pulmonary arterial smooth muscle cells; PHD2, prolyl hydroxylase‐2.