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. 2019 Mar 1;8(5):e011628. doi: 10.1161/JAHA.118.011628

Figure 5.

Figure 5

Effects of 2ME2 on female hPASMCs. HIF1α (A) and hexokinase 2 (HK2) (B) protein expression in 2ME2 (10 μmol/L; 48 hours) treated female hPASMCs. Bax (C) and RIPK1 (D) mRNA transcript expression in 2ME2 (10 μmol/L; 48 hours) treated female hPASMCs. (n=4–5). ‎CellEvent Caspase 3⁄7 Green activity in female hPASMCs after 6‐hour stimulation with 2ME2 (E) (10 μmol/L; n=3). Data are shown as mean±SEM. *P<0.05; **P<0.01; as indicated. Statistical analysis: (A through D) determined by paired t test; Panel E determined by 1‐way ANOVA with post hoc Tukey's test. Membranes were cut at an appropriate point and probed for separate antibodies. Membranes were then stripped and reprobed with β‐actin. 2ME2 indicates 2‐methoxyestradiol; Bax, Bcl‐associated X; CSS, charcoal‐stripped serum; HIF1α, hypoxia‐inducible factor‐1‐α; hPASMCs, human pulmonary arterial smooth muscle cells; RIPK1, receptor‐interacting serine/threonine–protein kinase 1; Veh, vehicle.