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. 2018 Oct 4;7(20):e010013. doi: 10.1161/JAHA.118.010013

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© 2018 The Authors. Published on behalf of the American Heart Association, Inc., by Wiley.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Antibody validation. A, Western blot showing transforming growth factor β receptor II (Tgfbr2) protein expression in the protein lysates from nontransgenic (Ntg) hearts and cardiomyocyte‐specific Tgfbr2‐ablated mice (R2^CD). B, Validation of antibodies for phospho‐SMAD3 (p‐SMAD3, phospho‐TAK1 (p‐TAK1) and phospho‐p38 (p‐p38) after treatment of fibroblasts with TGF‐β (+). GAPDH indicates glyceraldehyde 3‐phosphate; SMAD3, small mothers against decapentaplegic homolog 3; TAK1, transforming growth factor beta–activated kinase 1; TGF‐β, transforming growth factor beta.