Figure 13.

Ablation of transforming growth factor β receptor II gene Tgfbr2 (r2) in myofibroblasts does not alter misfolded protein accumulation in mutant αB‐crystallin (CryAB^{R 120G}) hearts. A, Immunofluorescent staining of CryAB (green) with α‐actinin (red) shows no difference in aggregate accumulations in cardiomyocytes derived from Cry AB^{R 120G} /Postnmcm/r2 ^f/f hearts with (+) or without (−) r2 ablation in the myofibroblasts (n=4). B and C, Western blot showing CryAB protein levels present in soluble and insoluble (aggregate‐containing) fractions. D and E, Quantitation of CryAB protein expression in soluble and insoluble fractions derived from hearts of the different experimental cohorts as indicated. CryAB was not detected in fractions derived from animals not expressing the CryAB^{R 120G}. Comparisons were made using 1‐way ANOVA followed by Tukey's post hoc test (n=4). *P<0.05 vs Cre induced Cry AB^{R 120G} /Postnmcm/r2 ^f/f. GAPDH indicates glyceraldehyde 3‐phosphate; Ntg, nontransgenic.