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. 2018 Oct 11;7(20):e009775. doi: 10.1161/JAHA.118.009775

Figure 4.

Figure 4

Sigmar1−/− hearts develop cardiac fibrosis at 6 months of age. A, Representative micrographs of Masson trichrome–stained LV myocardium and quantification of the cardiac fibrosis in Wt and Sigmar1−/− hearts (n=5 mice per group). Scale bars 50 μm. B, Representative micrographs of Sirius red–stained LV myocardium and quantification of the collagen deposition in Wt and Sigmar1−/− hearts (n=5 mice per group). Scale bars 50 μm. C, Representative Western blot and densitometric quantification showing significantly increased expression of periostin in Sigmar1−/− hearts (n=5 mice per group). D, Heart weight–to–body weight (HW/BW) ratio (n=7 mice per group), and mRNA expression of (E) natriuretic peptide A (Nppa), (F) natriuretic peptide B (Nppb), and (G) α‐myosin heavy chain (Myh6). Values are expressed as fold change vs Wt control (n=6 per group). H, Representative micrographs of wheat germ agglutinin (WGA, green)–stained LV myocardium and quantification of cross‐sectional area of the cardiomyocytes were carried on 10 microscoscopic fields for each heart (n=4 mice per group). Scale bars 50 μm. Bars represent mean±SEM. P values were determined by Tukey post hoc test. LV indicates left ventricle; NS, not significant; Wt, wild type.