Figure 5.

Genes involved in growth‐related pathways and cardiomyopathy are differentially regulated after 24 hours of dexamethasone treatment. A, Graph represents genes that are differentially regulated at 24 hours, along with the corresponding changes at 1 hour of dexamethasone treatment in cardiomyocytes (gene list provided as Table S3). B, Functional annotation of genes regulated only after 24 hours of dexamethasone treatment were analyzed using DAVID, and relevant groups with number of genes and P value are shown (all categorized groups shown in Figure S6). C, Transcript abundance of selected genes as measured by qPCR in neonatal ventricular cardiomyocytes treated with dexamethasone for 1, 6, or 24 hours. Error bars represent SEM, *P<0.05 compared with control, n=3. D, Integrated genomics viewer screenshots of selected representative genes with RNAseq and GR ChIP‐Seq data, showing change in the transcript after 1 and 24 hours of dexamethasone along with genomic GR binding status of these genes at 1 hour of dexamethasone treatment compared with control (ethanol) cardiomyocytes. ChIP‐Seq indicates chromatin immunoprecipitation sequencing; DAVID, Database for Annotation, Visualization and Integrated Discovery; Dex, dexamethasone; GR, glucocorticoid receptor; qPCR, quantitative polymerase chain reaction; RNAseq, RNA sequencing.