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. Author manuscript; available in PMC: 2019 Apr 20.
Published in final edited form as: Dig Liver Dis. 2016 Nov 17;49(2):188–196. doi: 10.1016/j.dld.2016.11.008

Figure 3: Mice treated with CD98 siRNA-loaded NPs (twice weekly by tail vein injection) show significant liver NP uptake and decreased lipid vacuolization when fed a high fat diet for 8 weeks.

Figure 3:

(A, B) Liver pictures of mice injected with scrambled siRNA loaded NPs (5mg/mL, 100μL) (A) and FITC tagged siRNA-loaded NPs (5mg/mL, 100μL) (B). After intravenous injection of FITC tagged siRNA-loaded NPs (5mg/mL, 100μL) (green), mice were sacrificed 4 h later and liver samples were stained for Alexa Fluor 568 phalloidin (red) and DAPI (blue). Representative hematoxylin and eosin (D, F) and Sirius Red (C, E)-stained sections of liver from 8 weeks high far diet fed mice receiving respectively scrambled siRNA-loaded NPs (5mg/mL, 100μL) (C, D) or CD98 siRNA-loaded NPs (5mg/mL, 100μL) (E, F) for 12 weeks. Scale bar is 50 μm. n = 5 mice per group. Hematoxylin and Eosin Y stained liver tissue was scored using the scoring system developed by Kleiner et al 34 of mice injected twice weekly with NP treatments (5mg/mL, 100μL) (G).