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. Author manuscript; available in PMC: 2019 Apr 20.
Published in final edited form as: Dig Liver Dis. 2016 Nov 17;49(2):188–196. doi: 10.1016/j.dld.2016.11.008

Figure 4: CD98 siRNA-loaded NPs reduce the levels of pro-steatosis markers (blood ALT and lipids) associated with the high fat diet fed mice and suppress CD98 protein expression level of CD98.

Figure 4:

(A) Serum ALT levels of mice maintained for 8 weeks on regular diet with injection of scrambled siRNA-loaded NPs (5mg/mL, 100μL) (regular diet + scrambled siRNA NP) or high fat diet (8 weeks) with twice a week injections of CD98 siRNA NPs (5mg/mL, 100μL) (HFD + siRNA CD98 NP) or scrambled siRNA-loaded NPs (5mg/mL, 100μL) (HFD + scrambled siRNA NP). (B) Liver triglyceride, cholesterols, high density lipoprotein cholesterol (HDLC) and glucose levels of mice maintained for 8 weeks on regular diet with injection of scrambled siRNA-loaded NPs (5mg/mL, 100μL) (regular diet + scrambled siRNA NP) or high fat diet (8 weeks) with twice a week injections of CD98 siRNA –loaded NPs (5mg/mL, 100μL) (HFD + siRNA CD98 NP) or scrambled siRNA-loaded NPs (5mg/mL, 100μL) (HFD + scrambled siRNA NP). Values represent means ± SE. Data are representative of n=5 mice per group. **P < 0.01, *P < 0.05 and NS, not statically significant. (C) Assessment of CD98 in liver from n mice (n being the number of mice used for each group as indicated by the different lines of the WB) fed with regular diet and injected twice a week with scrambled siRNA loaded nanoparticles (5mg/mL, 100μL) (regular diet + scrambled siRNA NP) or with high fat diet for 8 weeks and injected twine a week with CD98 siRNA loaded nanoparticles (5mg/mL, 100μL) (HFD + siRNA CD98 NP) or scrambled siRNA loaded NP (5mg/mL, 100μL) (HFD + scrambled siRNA NP) during 8 weeks. HFD + CD98 siRNA-NPs (n=5), HFD + scrambled siRNA-NPs (n=5), and RD + scrambled siRNA-NPs (n=4). n represent the number of mice for each group.