Representative FACS plots illustrating the gating strategy utilized to define live cells (A), macrophages like cells (B), dendritic cells (C) and hepatocytes (D) for mice biweekly injected with FITC-loaded NPs (5mg/mL, 100μL) and receiving or not HFD. Cells were gated as followed for hepatocytes CD45-MHC class II−F4/80−CD11b-CD11c-albumin+, macrophages-like cells CD45+MHC class II+F4/80+CD11b+CD11c- cells and dendritic cells CD45+MHC class II+ F4/80−CD11b+CD11c+. Values represent means ± SE. Data are representative of n=5 mice per group. **P < 0.01, *P < 0.05 and NS, not statically significant.