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. Author manuscript; available in PMC: 2020 Jan 10.
Published in final edited form as: Cell. 2018 Dec 27;176(1-2):167–181.e21. doi: 10.1016/j.cell.2018.10.053

Figure 2. ssDNA downstream of an intact DPC facilitates CMG bypass.

Figure 2.

The indicated plasmids were pre-incubated with LacR, replicated in SPRTN-depleted egg extract, and analyzed as in Figure S1J. Approach was used as a proxy for CMG bypass and quantified as in Figure 1C (see methods). Figure S2 depicts the proposed events on each plasmid. The mean of n=3 experiments is graphed. Error bars represent the standard deviation.