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. 2019 Apr 20;39:7. doi: 10.1186/s41232-019-0097-x

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Effect of various concentrations of ethanol (a) and methanol (b) extracts of Calendula the expression of TGFβ1 protein in the MEFs culture supernatants. The MEFs were treated with different concentration of ethanol and methanol extracts of Calendula (5 and 10 μg/ml) at different time intervals treatment (12 and 24 h) and the expression of TGFβ1 protein was assessed by ELISA