Table 3.

The total expression ratio of the gene of TGFβ1 in the MEFs treated with various concentrations of ethanol extract of Calendula (5 and 10 μg/ml) relative to control group is presented in each time (12 and 24 h after treatment). The statistic test for significance is randomization re-allocation test, implemented in the relative expression software tool. Significant down or upregulations of the genes highlighted

	12 h after treatment		24 h after treatment
	5 μg/ml	10 μg/ml	5 μg/ml	10 μg/ml
Relative expression	1.7	2.3	0.7	0.62
Standard error	± 0.21	± 0.28	± 0.24	± 0.16
P value	0.235	0.001	0.490	0.359
Fold increase/decrease	+ 1.7	+ 2.3	− 1.4	− 1.6