Extended Data Fig. 6 |. The longest stretch of IDR in DYRK1A promotes formation of phase-separated droplets in an HRD-dependent manner.

a, Intrinsic disorder tendency was predicted by IUPred across the entire length of DYRK1A. The scores are assigned between 0 and 1, and a score above 0.5 indicates disorder. The HRD region is shaded in blue. The longest stretch of IDR is labelled as DYRK1A-IDR and its boundaries are marked. b, The indicated C-terminally Strep-tagged GFP fusions were purified from recombinant E. coli BL21 cells. Two micrograms each of the fusion proteins was examined by SDS–PAGE followed by Coomassie blue staining. c, Solutions containing 5 mg ml⁻¹ of the indicated fusion proteins, 37.5 mM NaCl and 10% PEG8000 were trapped between coverslips and examined with a microscope under either fluorescent (488 nm) or normal white light (brightfield).